1. Send me an equery, get a negative response, then invite me to your myspace page.
result: your email address tagged as junk, and diverted automatically to the trash forevermore.
2. Put the words "hot" "sex" or "porn" in your subject line.
My email program is blindingly stupid as only a machine can be. It thinks you're spam and you've been diverted to the spam folder. When I wade into the spam folder I'm looking for email addresses I recognize and subject lines I know. Yours misses on both
3. Send attachments. I don't read or open attachments unless I've asked to see the material. If you query me with an email that says "I've attached my query letter" I won't read it and I will send you a form rejection. Policies vary agency by agency on this but mine is as clear as I can make it. NO ATTACHMENTS on query letters.